Charges for the injection service from Karolinska Center for Transgene
Technologies
Because the KCTT is a non-business,
non-profit academic research institution, we ask the groups using our
service to reimburse our expenses like media, animals used, salary costs,
technical costs etc. KCTT is working at a cost recovery basis.
Due to the increased costs
for personnel, equipment and animals as also to the reduced funding, KCTT
is adjusting the charges for its services from January 1st 2012. We hope that
the groups working together with us do understand this step.
Due to the Swedish law,
the KI is obliged to charge also VAT. If you have a VAT number at your
institution, please don't forget to write it down in the application form. VAT
will then not be charged. If you do not have a VAT number VAT can be refunded
by the Swedish state. The prices for non-KI customers are including overhead
but excluding VAT.
The costs are as follows for the
different services:
|
Services |
Service Includes |
Price for KI Research Groups* |
Price for non-KI Research Groups |
|
Pronucleus Injection - Pups weaned |
One-day pronuclear injection of one DNA construct in hybrid B6D2F1 or C57BL/6N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
16 017 SEK |
19 576 SEK |
|
Pronucleus Injection – Pups taken as embryos |
One-day pronuclear injection of one DNA construct in hybrid B6D2F1 or C57BL/6N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals analyzed by customer during embryo stages. |
15 369 SEK |
18 784 SEK |
|
Pronucleus Injection in C57BL/6J mice – Pups weaned |
One-day pronuclear injection of one DNA construct in C57BL/6J mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
19 944 SEK |
24 376 SEK |
|
Pronucleus Injection in FVB/N mice – Pups weaned |
One-day pronuclear injection of one DNA construct in FVB/N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
20 224 SEK |
24 719 SEK |
|
Pronucleus Injection of BAC / PAC / YAC constructs – Pups weaned |
Two days pronuclear injection of one DNA construct in C57BL/6N or hybrid B6D2F1 mice (DNA cleaned by customer; injection of at least 210 embryos in total). Due to the higher lethality after injection the amount of transfers can not be guaranteed. |
28 018 SEK |
34 245 SEK |
|
Preparation of DNA construct |
Cleavage and cleaning of normal DNA constructs (non P1, BAC, PAC plasmids) for pronuclear injection. |
6 828 SEK |
8 345 SEK |
|
ES Cell Injection |
One-day injection of one clone. Injection does not include the preparation of the ES cells for the injection. Produced chimeras will be delivered directly after weaning. |
15 903 SEK |
19 436 SEK |
|
Electroporation |
The price includes the electroporation, the picking and freezing of clones surviving selection, and preparation of ES cell lystates for screening. The price does not include shipping and screening of the clones. |
22 629 SEK |
27 658 SEK |
|
ES Cell Clone Preparation |
Preparation for the ES cell clone prior to the injection. |
3 009 SEK |
3 678 SEK |
|
Re-Derivation of Infected Mouse Strains
|
Re-derivation of mice from frozen embryos. The service is done in collaboration with the Import Portal North. The price does not include the shipping costs for the frozen embryos. |
7 761 SEK |
9 486 SEK |
Re-Derivation of Infected Mouse Strains
|
Cleaning of infected mouse strains. The service is done in
collaboration with the Import Portal North. The price does not include the
costs for the mice used and for housing the mice prior to re-derivation.
Please contact Portalen for more information. |
10 701 SEK |
13 078 SEK |
Spermfreezing |
Freezing of sperms. The service is done in collaboration with the Import Portal North. The price does not include the costs for the mice used and for housing the mice prior to freezing. Please contact Portalen for more information. |
6 485 SEK |
7 926 SEK |
|
Freezing of Embryos (natural mating)
|
Freezing of 2 cell stage embryos. The service is done in collaboration with the Import Portal North. The price does not include the costs for the mice used and for housing the mice prior to freezing. Please contact Portalen for more information. |
7 650 SEK |
10 328 SEK |
In vitro fertilization (IVF) with embryo freezing |
In vitro fertilization of mouse strains followed by embryo freezing. The service is done in
collaboration with the Import Portal North. The price does not include the
costs for the mice used and for housing the mice prior to re-derivation.
Please contact Portalen for more information. |
9 639 SEK |
11 847 SEK |
In Vitro Fertilization (IVF) with following Re-Derivation |
In vitro fertilization of mouse strains followed by a re-derivation (embryo transfer). The service is done in
collaboration with the Import Portal North. The price does not include the
costs for the mice used and for housing the mice prior to IVF and re-derivation.
Please contact Portalen for more information. |
10 139 SEK |
17 236 SEK |
In Vitro Fertilization (IVF) with Re-Derivation and Embryo Freezing |
In vitro fertilization of mouse strains with embryo freezing and re-derivation (embryo transfer). The service is done in collaboration with the Import Portal North. The price does not include the costs for the mice used and for housing the mice prior to IVF and re-derivation. Please contact Portalen for more information. |
12 780 SEK |
19 169 SEK |
Storage of frozen embryos / sperms |
Storage of frozen embryos or sperms in LN2 cryotanks. Price per frozen strain. Max. charge 2000 SEK / group (KI) or 2800 (external). | 500 SEK |
700 SEK |
NOTE!
All prices given above without INDI!
VAT:
All
prices given above without VAT.
Please provide KCTT with your VAT number, so that VAT does not have to be
added.
Maintenance of mice:
If mice can
not be transferred to the customer KCTT can not guarantee space for
continued housing. If space is available in the animal facility, the costs for
housing will be charged to the customer. If no space is available, the customer
will be asked to take care about the produced mice within the next 4 weeks.
After this period the mice will be euthanized. All accrued costs will be
charged to the customer.
Shipping of mice:
The full
shipping costs for the produced animal will be charged to the customer.
Shipping of clones:
The full shipping
costs of the ES cells from and to the customer will be charged to the customer.