Charges for the injection service from Karolinska Center for Transgene
Technologies
Because the KCTT is a non-business,
non-profit academic research institution, we ask the groups using our
service to reimburse our expenses like media, animals used, salary costs,
technical costs etc. KCTT is working at a cost recovery basis.
Due to the increased costs
for personnel, equipment and animals as also to the reduced funding, KCTT
is adjusting the charges for its services from April 1st 2010. We hope that
the groups working together with us do understand this step.
Due to the Swedish law,
the KI is obliged to charge also VAT. If you have a VAT number at your
institution, please don't forget to write it down in the application form. VAT
will then not be charged. If you do not have a VAT number VAT can be refunded
by the Swedish state. The prices for non-KI customers are including overhead
but excluding VAT.
The costs are as follows for the
different services:
|
Services |
Service Includes |
KI Price without Internal Overhead |
KI Price with Internal Overhead |
KI Price for non-KI Research Groups |
|
Pronucleus Injection - Pups weaned |
One-day pronuclear injection of one DNA construct in hybrid B6D2F1 or C57BL/6N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
16 725 SEK |
20 907 SEK |
22 579SEK |
|
Pronucleus Injection – Pups taken as embryos |
One-day pronuclear injection of one DNA construct in hybrid B6D2F1 or C57BL/6N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals analyzed during embryo stages. |
16 005 SEK |
20 007 SEK |
21 607 SEK |
|
Pronucleus Injection in C57BL/6J mice – Pups weaned |
One-day pronuclear injection of one DNA construct in C57BL/6J mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
22 857 SEK |
28 572 SEK |
30 857 SEK |
|
Pronucleus Injection in FVB/N mice – Pups weaned |
One-day pronuclear injection of one DNA construct in FVB/N mice. DNA cleaned by the customer. Injection of embryos for at least 6 transfers in pseudopregnant females; animals delivered directly after weaning. |
20 882 SEK |
26 103 SEK |
28 191 SEK |
|
Pronucleus Injection of BAC / PAC / YAC constructs – Pups weaned |
Two days pronuclear injection of one DNA construct in C57BL/6N or hybrid B6D2F1 mice (DNA cleaned by customer; injection of at least 210 embryos in total). Due to the higher lethality after injection the amount of transfers can not be guaranteed. |
30 356 SEK |
37 945 SEK |
40 981 SEK |
|
Preparation of DNA construct |
Cleavage and cleaning of normal DNA constructs (non P1, BAC, PAC plasmids) for pronuclear injection. |
7 586 SEK |
9 483 SEK |
10 242 SEK |
|
ES Cell Injection |
One-day injection of one clone. Injection does not include the preparation of the ES cells for the injection. Produced chimeras will be delivered directly after weaning. |
19 485 SEK |
24 357 SEK |
26 305 SEK |
|
Electroporation |
The price includes the electroporation, the picking and freezing of clones surviving selection, and preparation of ES cell lystates for screening. The price does not include shipping and screening of the clones. |
25 018 SEK |
31 273 SEK |
33 775 SEK |
|
ES Cell Clone Preparation |
Preparation for the ES cell clone prior to the injection. |
4 054 SEK |
5 068 SEK |
5 473 SEK |
|
Re-Derivation of Infected Mouse Strains |
Cleaning of infected mouse strains. The service is done in collaboration with the Import Portal North. The price does not include the costs for the mice used and for housing the mice prior to re-derivation. Please contact KCTT for more information. |
7 282 SEK |
9 103 SEK |
9 831 SEK |
|
Freezing of Embryos |
Freezing of 2 cell stage embryos. The service is done in collaboration with the Import Portal North. The price does not include the costs for the mice used and for housing the mice prior to freezing. Please contact KCTT for more information. |
7 650 SEK |
9 563 SEK |
10 328 SEK |
NOTE!
VAT:
All
prices given above without VAT.
Please provide KCTT with your VAT number, so that VAT does not have to be
added.
Maintenance of mice:
If mice can
not be transferred to the customer KCTT can not guarantee space for
continued housing. If space is available in the animal facility, the costs for
housing will be charged to the customer. If no space is available, the customer
will be asked to take care about the produced mice within the next 4 weeks.
After this period the mice will be euthanized. All accrued costs will be
charged to the customer.
Shipping of mice:
The full
shipping costs for the produced animal will be charged to the customer.
Shipping of clones:
The full shipping
costs of the ES cells from and to the customer will be charged to the customer.