ACCESS
The Karolinska Center for Transgene Technologies (KCTT) distinguishes between the following categories of customers:
1. Research Groups from KI
2. Academic Groups non-KI
Companies that want to use the service from KCTT have to get in contact to Karolinska Research Services AB for a more detailed information of the service offered.
Waiting List
1. KI customers
The orders from Karolinska Institute, Karolinska Hospital, Huddinge Hospital and other KI-connected research groups will be treated on a strict "first come, first served" basis, i.e. the place in the waiting list will be based on the day KCTT receives the DNA construct. The aim is that the time between receipt of the DNA construct and injection into fertilized mouse eggs should not exceed 1 month (for the pronuclear injection technique). For electroporations the time between receipt of the DNA construct and the electroporation into ES cells should not exceed 2 months. For the injection of ES cells, targeted by the research groups, the waiting time should not be longer than 6 - 8 weeks.
2. Academic groups (non-KI)
Like for KI groups, the waiting time for pronucleus injection, electroporation and injection of own targeted ES cells should be in the range of the time given for KI groups. Besides this KCTT can't promise to keep the place in the waiting list in case urgent constructs from KI groups have to be injected. Before changing a date, KCTT will set all efforts to keep the promised schedule.
To place an order for the electroporation, the generation of transgenic mice by the pronuclear injection or ES cell technique, the respective ordering forms should be used and sent to KCTT. The appendices, belonging to the different forms, should be sent along with the application forms. Incomplete applications cannot be accepted.
It is desirable that the research group notifies KCTT as early as possible,
to indicate that they plann to use the services from KCTT in the near future.
This greatly facilitates the long-term planning for KCTT.
It is normally not possible to pre-book an injection date, only in case of time
sensitive experiments have to be done (e.g. taking out embryos at a certain
stage, injection of large constructs) dates can be booked. If a time point for
the injection has been set, the DNA construct has to be delivered latest 2 weeks
before the date. If the DNA is not delivered in time or the injection is cancelled
too late, the costs for the whole injection date will be charged to the group.
KCTT is working on a cost recovery basis and therefore will charge the groups
using the service only the arising costs! In addition the Karolinska Institute
is forced to take VAT and overhead from non-KI customers. VAT can be reclaimed
from the Swedish tax office. The administration will help you in case of questions.
An Advisory Board has been appointed by the Board of Research to review the activities of KCTT..
The management of KCTT is composed of Johannes Wilbertz (Director), situated at the Campus-North and Stephan Teglund (Vice-Director), situated at Campus-South. Together with the chairman of the scientific board they are heading the activities.
The staff from the KCTT carries out the pronuclear and blastocyst injections as well as all other techniques in the transgenic field. The ES cell culturing is carried out by the technicians of the ES Cell Lab at KCTT. In addition, personnel from the animal facilities provide help with the animal care taking, superovulation, plug checks etc.
Each research group is responsible for obtaining an approval
from the local ethical committee for the transgenic experiment.
Together with the application form and the respective appendix for pronucleus
or blastocyst injection, the research group has to send in a copy of their ethical
permission. Without this copy and the signed forms KCTT can not start
the injections.
Information about projects carried out by KCTT will be treated as confidential and not released to a third party.
For discussing the possibility of patenting a transgenic mouse strain, please consult with Karolinska Innovations AB.
Please consult Johannes Wilbertz or Stephan Teglund if further information is needed.
14-Apr-2008